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universal primers hybridize m13-reverse t7 promoter sequences  (Promega)

 
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    Structured Review

    Promega universal primers hybridize m13-reverse t7 promoter sequences
    Universal Primers Hybridize M13 Reverse T7 Promoter Sequences, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/universal+primers+hybridize+m13-reverse+t7+promoter+sequences/universal+primers+hybridize+m13+reverse+t7+promoter+sequences/10__1074_slash_jbc__m200149200-76-17-22
    Average 90 stars, based on 1 article reviews
    universal primers hybridize m13-reverse t7 promoter sequences - by Bioz Stars, 2026-09
    90/100 stars

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    Sequencing:

    Article Title: The Salmonella typhimurium Flagellar Basal Body Protein FliE Is Required for Flagellin Production and to Induce a Proinflammatory Response in Epithelial Cells
    Article Snippet: To determine the site of transposon insertion, an EcoRI digest was performed, and the Kan fragment was ligated into pBluescript (Stratagene, La Jolla, CA), and the resulting constructs were transformed into XL1-Blue (Stratagene). .. The nucleotide sequence of DNA flanking the transposon was determined using universal primers that hybridize to the M13-reverse and T7 promoter sequences (Promega Corp., Madison, WI) or to 19- and 20-bp regions of the Kan resistance cassette (5 -GCTTCCATCCGAGTACGTG, 5 -GCTCGACGTTGTCACTGAAG). .. Sequencing chemistry was performed using an ABI PRIZM “Big Dye” terminator cycle sequencing ready reaction kit with amplitaque FS, and analysis was carried out on an ABI PRIZM 377 automated DNA sequencer (Applied Biosystems/PerkinElmer Life Sciences).



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    Promega universal primers hybridize m13-reverse t7 promoter sequences
    Universal Primers Hybridize M13 Reverse T7 Promoter Sequences, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/universal+primers+hybridize+m13-reverse+t7+promoter+sequences/universal+primers+hybridize+m13+reverse+t7+promoter+sequences/10__1074_slash_jbc__m200149200-76-17-22
    Average 90 stars, based on 1 article reviews
    universal primers hybridize m13-reverse t7 promoter sequences - by Bioz Stars, 2026-09
    90/100 stars
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